Sterile technique basics for agar, grain, and grow bags

Sterile technique is simple in theory: keep contamination out while you work. The hard part is staying patient.

Before you start

• Clean the table.
• Set up your SAB or flow hood if you use one.
• Wipe tools and surfaces.
• Keep sterile items sealed until the moment you use them.

While you work

• Move slowly.
• Do not wave your hands over open plates, jars, or bags.
• Do one step at a time.
• If something looks wrong, stop and reset.

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